rabbit polyclonal igg to stat3  (Proteintech)

Journal: Molecular Medicine Reports

Article Title: ST3Gal1 modulates intestinal barrier function and impacts human ulcerative colitis

doi: 10.3892/mmr.2025.13783

Figure Lengend Snippet: ST3Gal1 expression in inflamed IEC monolayers alters MUC2, TFF3, CDX2, STAT3 and p-STAT3 expression. ST3Gal1 knockdown in ST3Gal1-sh3/IEC significantly increased (A) MUC2, (B) TFF3 and (C) CDX2, but significantly reduced (D) STAT3 mRNA levels in the inflamed IEC monolayer. ST3Gal1 OE in ST3Gal1-OE/IEC significantly reduced (E) MUC2, (F) TFF3 and (G) CDX2 mRNA levels in the inflamed IEC monolayer. (H) ST3Gal1 OE in ST3Gal1-OE/IEC slightly decreased STAT3 mRNA levels in the inflamed IEC monolayer. (I) Western blotting showed no changes in protein expression in non-inflamed knockdown samples. (J) Western blotting showed that MUC2, TFF3 and CDX2 protein levels were increased, and p-STAT3 expression was decreased in the inflamed IEC monolayer comprising ST3Gal1-sh3/IEC. (K) Western blotting showed no notable changes in protein expression in non-inflamed OE samples. (L) Western blotting showed that MUC2, TFF3 and CDX2 protein levels were decreased, and p-STAT3 expression was increased in the inflamed IEC monolayer comprising ST3Gal1-OE/IEC. Data are presented as the mean ± SD. Statistical significance was assessed using one-way analysis of variance followed by Tukey's post-hoc test. *P<0.05 and **P<0.01. IEC, intestinal epithelial cell; sh, short hairpin; Ctr, control; OE, overexpression; MUC2, mucin 2; TFF3, trefoil factor 3; CDX2, homeobox protein CDX-2; p-, phosphorylated.

Article Snippet: Rabbit polyclonal IgG to STAT3 , Proteintech Group, Inc. , 10253-2-AP , 1:500.

Techniques: Expressing, Knockdown, Western Blot, Control, Over Expression

Journal: Molecular Medicine Reports

Article Title: ST3Gal1 modulates intestinal barrier function and impacts human ulcerative colitis

doi: 10.3892/mmr.2025.13783

Figure Lengend Snippet: Comparison of the expression of inflammatory mediators (A) IL-1β, (B) IL-6 and (C) IL-8, as well as (D) STAT3 between non-lesional mucosa (n=44) and lesional mucosa (n=75) from distinct patients with ulcerative colitis. Correlations between (E) IL-1β, (F) IL-6, (G) IL-8 and (H) STAT3 mRNA levels and ST3Gal1 expression. Data were obtained from the GSE107499 dataset from the Gene Expression Omnibus database. Data in A-D are presented as box and whisker plots, and those in E-H as scatter plots. Statistical significance for group comparisons in A-D was assessed using the unpaired t-test; correlation analyses in E-H were performed via Pearson's correlation coefficient. **P<0.01.

Article Snippet: Rabbit polyclonal IgG to STAT3 , Proteintech Group, Inc. , 10253-2-AP , 1:500.

Techniques: Comparison, Expressing, Gene Expression, Whisker Assay

Journal: Stem Cells International

Article Title: Pluripotency of Dental Pulp Cells and Periodontal Ligament Cells Was Enhanced through Cell-Cell Communication via STAT3/Oct-4/Sox2 Signaling

doi: 10.1155/2021/8898506

Figure Lengend Snippet: Differentially expressed genes in DPCs and PDLCs with indirect coculture. The x -axis (a log transformation plot of the fold difference) and the y -axis ( P value) indicate the differential expression of each gene in the indirect coculture and control groups of DPCs (a) and PDLCs (b). The pink lines indicate a 3-fold up- or downregulation of gene expression. The blue line indicates the threshold for the P value ( P < 0.05) of the t -test. In the indirect coculture systems, four genes ( ZFPM2 , STAT3 , SOX2 , and OCT-4 ) were significantly upregulated, and seven genes ( EGR3 , PAX5 , PCNA , STAT1 , RUNX1 , FGF1 , and NOTCH2 ) were significantly downregulated in the indirect cocultured DPCs. Meanwhile, ten genes ( STAT3 , HOXC10 , HOXA9 , EZH2 , ESR1 , SOX2 , OCT-4 , DLX2 , PPARG , and KLF4 ) were significantly upregulated, whereas four genes ( PAX1 , NANOG , HOXA7 , and PAX5 ) were significantly downregulated in the indirect cocultured PDLCs. Western blot analyses showed that the levels of STAT3, OCT-4, and SOX2 protein expression were strongly upregulated and PAX5 expression was downregulated in DPCs (c, d) and PDLCs (e, f) after indirect coculture.

Article Snippet: Next, the chromatin was incubated with anti-STAT3 rabbit polyclonal antibody (Cell Signaling Technology, Danvers, MA, USA) or normal rabbit IgG (Cell Signaling Technology) overnight at 4°C.

Techniques: Transformation Assay, Quantitative Proteomics, Control, Gene Expression, Western Blot, Expressing

Journal: Stem Cells International

Article Title: Pluripotency of Dental Pulp Cells and Periodontal Ligament Cells Was Enhanced through Cell-Cell Communication via STAT3/Oct-4/Sox2 Signaling

doi: 10.1155/2021/8898506

Figure Lengend Snippet: List of genes with altered expression with DPCs with indirect coculture.

Article Snippet: Next, the chromatin was incubated with anti-STAT3 rabbit polyclonal antibody (Cell Signaling Technology, Danvers, MA, USA) or normal rabbit IgG (Cell Signaling Technology) overnight at 4°C.

Techniques: Expressing, Functional Assay

Journal: Stem Cells International

Article Title: Pluripotency of Dental Pulp Cells and Periodontal Ligament Cells Was Enhanced through Cell-Cell Communication via STAT3/Oct-4/Sox2 Signaling

doi: 10.1155/2021/8898506

Figure Lengend Snippet: List of genes with altered expression with PDLCs with indirect coculture.

Article Snippet: Next, the chromatin was incubated with anti-STAT3 rabbit polyclonal antibody (Cell Signaling Technology, Danvers, MA, USA) or normal rabbit IgG (Cell Signaling Technology) overnight at 4°C.

Techniques: Expressing, Functional Assay

Journal: Stem Cells International

Article Title: Pluripotency of Dental Pulp Cells and Periodontal Ligament Cells Was Enhanced through Cell-Cell Communication via STAT3/Oct-4/Sox2 Signaling

doi: 10.1155/2021/8898506

Figure Lengend Snippet: Expression and distribution of stem cell-related markers in the rat defect models. Hematoxylin and eosin staining revealed the structures of the normal and injury groups (a, b). The primary antibody was replaced by PBS in the negative control groups of normal and injury groups (c, d). STAT3, Oct-4, and Sox2 were notably present in the nucleus and, to a lesser extent, in the cytoplasm of the injury groups (f, h, and j). Rare staining of STAT3, Oct-4, and Sox2 was found in the normal groups (e, g, and i). Intense staining of PAX5 was found in the cytoplasm and matrix around the normal dental pulp and periodontal ligament when compared with PAX5 staining in the repaired connective tissues (k). Rare staining of PAX5 was detected in the injury groups (l). Scale bars = 100 μ m. D: dentin; DP: dental pulp; PDL: periodontal ligament.

Article Snippet: Next, the chromatin was incubated with anti-STAT3 rabbit polyclonal antibody (Cell Signaling Technology, Danvers, MA, USA) or normal rabbit IgG (Cell Signaling Technology) overnight at 4°C.

Techniques: Expressing, Staining, Negative Control

Journal: Stem Cells International

Article Title: Pluripotency of Dental Pulp Cells and Periodontal Ligament Cells Was Enhanced through Cell-Cell Communication via STAT3/Oct-4/Sox2 Signaling

doi: 10.1155/2021/8898506

Figure Lengend Snippet: STAT3 overexpression and silence models were established in DPCs and PDLCs. The results of PCR and WB showed that STAT3 was significantly enhanced and silenced in DPCs and PDLCs (a, b, e, and f, ∗∗∗ P < 0.001). Oct-4 (c, d) and Sox2 (c, d) showed similar patterns of mRNA expression, consistent with the overexpression or silence of STAT3 , and both mRNA levels were significantly upregulated or downregulated in both DPCs and PDLCs ( ∗ P < 0.05, ∗∗ P < 0.01, and ∗∗∗ P < 0.001). The results of western blot showed that the levels of Oct-4 and Sox2 proteins were significantly increased or reduced in DPCs and PDLCs (e, f). The apoptosis rates of DPCs and PDLCs were significantly reduced compared with the control group (g, , ∗∗ P < 0.01 and ∗∗∗ P < 0.001). The cell cycles of both DPCs and PDLCs were arrested at the G0/G1 phase (h, , ∗∗ P < 0.01). 1: group of control; 2: group of pcDNA3.0; 3: group of pcDNA3.0-STAT3; 4: group of shCTRL; and 5: group of shSTAT3.

Article Snippet: Next, the chromatin was incubated with anti-STAT3 rabbit polyclonal antibody (Cell Signaling Technology, Danvers, MA, USA) or normal rabbit IgG (Cell Signaling Technology) overnight at 4°C.

Techniques: Over Expression, Expressing, Western Blot, Control

Journal: Stem Cells International

Article Title: Pluripotency of Dental Pulp Cells and Periodontal Ligament Cells Was Enhanced through Cell-Cell Communication via STAT3/Oct-4/Sox2 Signaling

doi: 10.1155/2021/8898506

Figure Lengend Snippet: Apoptosis and cell cycle of PDCs and PDLCs in overexpression and silence models (mean ± SD%; N = 3).

Article Snippet: Next, the chromatin was incubated with anti-STAT3 rabbit polyclonal antibody (Cell Signaling Technology, Danvers, MA, USA) or normal rabbit IgG (Cell Signaling Technology) overnight at 4°C.

Techniques: Over Expression

Journal: Stem Cells International

Article Title: Pluripotency of Dental Pulp Cells and Periodontal Ligament Cells Was Enhanced through Cell-Cell Communication via STAT3/Oct-4/Sox2 Signaling

doi: 10.1155/2021/8898506

Figure Lengend Snippet: STAT3 bound to the endogenous Oct-4/Sox2 promoters. (a) Oligonucleotide sequences indicating the Oct-4/Sox2 promoters and the distance from the transcription start site (TSS) are shown. Green arrows denote the transcription direction. (b) The relative luciferase activities of the Oct-4 and Sox2 mutants were significantly decreased when compared with the STAT3 mimic in cotransfected 293 T cells. Data are represented as the mean ± SD. ∗ P < 0.05, ∗∗ P < 0.01, and ∗∗∗ P < 0.001; wild-type (WT) vs. mutation (MUT). (c) STAT3 binding to the endogenous Oct-4 and Sox2 promoters was evaluated by the chromatin immunoprecipitation assay. The levels of Oct-4 and Sox2 promoter regions in the lower chamber DPCs were significantly higher than those in the control group. (d) The binding sites for STAT3 on Oct 4 and Sox2 were detected by the electrophoretic mobility shift assay (EMSA) performed with DIG-labeled oligonucleotide probes (lane 2). Incubation with a specific competitor abolished the binding (lane 3), while addition of a mutant competitor increased the intensities of the putative bands (lane 4). Moreover, incubation with STAT3 antibody reduced the intensities of those bands while causing an upward super shift (lane 5).

Article Snippet: Next, the chromatin was incubated with anti-STAT3 rabbit polyclonal antibody (Cell Signaling Technology, Danvers, MA, USA) or normal rabbit IgG (Cell Signaling Technology) overnight at 4°C.

Techniques: Luciferase, Mutagenesis, Binding Assay, Chromatin Immunoprecipitation, Control, Electrophoretic Mobility Shift Assay, Labeling, Incubation

Journal: Journal of Neuroinflammation

Article Title: Activation of the inflammatory transcription factor nuclear factor interleukin-6 during inflammatory and psychological stress in the brain

doi: 10.1186/1742-2094-10-140

Figure Lengend Snippet: Dilutions for the primary antibodies used for immunohistochemistry or immunocytochemistry

Article Snippet: STAT3 , Rabbit polyclonal IgG , 1:8000 , cat. sc-21876; Santa Cruz Biotechnology, Santa Cruz, CA, USA.

Techniques: Immunohistochemistry